Hotspot

ccsA

SSC · 109332-110309 · 978 bp

Back to Acidosasa

Pi 0.000682

MarkerSeek score 46.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 1
pi: 0.000682
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AGTGGCGGCATTCTCAAAAA	TTTGAGAAGGCGTTCAAGGG	45.0	50.0	58.7	58.4	1144 bp	1.0	71.5
ccsA_p2	AGTGGCGGCATTCTCAAAAA	GGCGTTCAAGGGTTCTCAAA	45.0	50.0	58.7	58.7	1136 bp	1.0	69.2
ccsA_p3	AGTGGCGGCATTCTCAAAA	TTTGAGAAGGCGTTCAAGGG	47.4	50.0	58.0	58.4	1144 bp	1.0	68.6
ccsA_p4	GAGTGGCGGCATTCTCAAAA	TTTGAGAAGGCGTTCAAGGG	50.0	50.0	59.1	58.4	1145 bp	1.0	68.0
ccsA_p5	AGTGGCGGCATTCTCAAAAA	GAAGGCGTTCAAGGGTTCTC	45.0	55.0	58.7	58.8	1139 bp	1.0	67.9