Hotspot

atpI

LSC · 33052-33795 · 744 bp

Back to Acidosasa

Pi 0.000896

MarkerSeek score 46.80

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 1
pi: 0.000896
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	GCTGAGTTGAGAAAGAGATGGT	AGTTTTCCAACCAAGCGGAT	45.5	45.0	58.1	58.0	900 bp	1.0	63.5
atpI_p2	TGCTGAGTTGAGAAAGAGATGG	AGTTTTCCAACCAAGCGGAT	45.5	45.0	58.1	58.0	901 bp	1.0	63.5
atpI_p3	ACTTCAAGTTGCTGAGTTGAGA	AGTTTTCCAACCAAGCGGAT	40.9	45.0	57.9	58.0	910 bp	1.0	62.9
atpI_p4	ACTTCAAGTTGCTGAGTTGAG	AGTTTTCCAACCAAGCGGAT	42.9	45.0	56.4	58.0	910 bp	1.0	59.3
atpI_p5	GCTGAGTTGAGAAAGAGATGG	AGTTTTCCAACCAAGCGGAT	47.6	45.0	56.3	58.0	900 bp	1.0	58.8