Hotspot

atpB-rbcL

LSC · 56224-57003 · 780 bp

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Pi 0.000855

MarkerSeek score 46.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 1
pi: 0.000855
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTGATCGATACGCCCTGTG	CCTTGGTTTCATACTCCGGG	50.0	55.0	58.1	58.0	953 bp	1.0	79.3
atpB-rbcL_p2	TTTGATCGATACGCCCTGTG	CTTGGTTTCATACTCCGGGG	50.0	55.0	58.1	58.0	952 bp	1.0	79.3
atpB-rbcL_p3	TATCCAGCACGGGTCCAATA	CCTTGGTTTCATACTCCGGG	50.0	55.0	58.2	58.0	974 bp	1.0	78.1
atpB-rbcL_p4	TATCCAGCACGGGTCCAATA	CTTGGTTTCATACTCCGGGG	50.0	55.0	58.2	58.0	973 bp	1.0	78.1
atpB-rbcL_p5	CCAGCACGGGTCCAATAATT	CTTGGTTTCATACTCCGGGG	50.0	55.0	58.2	58.0	970 bp	1.0	77.9