Hotspot

atpH-atpF

LSC · 34872-35326 · 455 bp

Back to Acidosasa

Pi 0.00147

MarkerSeek score 56.90

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 1
pi: 0.00147
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	TGTGGCACTAGCGCTTTTAT	GGATGGCCAGTGAGCTAAAA	45.0	50.0	57.9	57.9	542-543 bp	1.0	78.4
atpH-atpF_p2	TTGTGGCACTAGCGCTTTTA	GGATGGCCAGTGAGCTAAAA	45.0	50.0	58.1	57.9	543-544 bp	1.0	78.3
atpH-atpF_p3	TGTGGCACTAGCGCTTTTAT	ATTAAGCCCGAAACTCCCAG	45.0	50.0	57.9	57.6	563-564 bp	1.0	76.1
atpH-atpF_p4	TTGTGGCACTAGCGCTTTTA	ATTAAGCCCGAAACTCCCAG	45.0	50.0	58.1	57.6	564-565 bp	1.0	76.0
atpH-atpF_p5	GTGGCACTAGCGCTTTTATT	GGATGGCCAGTGAGCTAAAA	45.0	50.0	56.8	57.9	541-542 bp	1.0	69.6