primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK_p1	matK	1	TCAGAAGAACATTTCAGAATGGA	ATGCAACACCCTGTTTTGAC	23	20	34.8	45.0	55.9	57.1	4.4	0.0	6.035549	1708	3243	1715-1715	1.000000	40.0
matK_p2	matK	2	TCAGAAGAACATTTCAGAATGGA	AATGCAACACCCTGTTTTGAC	23	21	34.8	42.9	55.9	57.8	4.4	0.0	6.324712	1708	3243	1716-1716	1.000000	40.0
matK_p3	matK	3	TCAGAAGAACATTTCAGAATGGA	GCAACACCCTGTTTTGACCA	23	20	34.8	50.0	55.9	59.2	4.4	0.0	6.325844	1708	3243	1713-1713	1.000000	40.0
matK_p4	matK	4	TCAGAAGAACATTTCAGAATGGA	TGCAACACCCTGTTTTGACC	23	20	34.8	50.0	55.9	59.2	4.4	0.0	6.325844	1708	3243	1714-1714	1.000000	40.0
matK_p5	matK	5	TCAGAAGAACATTTCAGAATGGAA	ATGCAACACCCTGTTTTGAC	24	20	33.3	45.0	56.5	57.1	4.4	0.0	6.345566	1708	3243	1715-1715	1.000000	40.0
rps16_p1	rps16	1	TGGCACGACATAAATCCAGT	TCCCTATACCACTTTGCAATGA	20	22	45.0	40.9	57.2	57.1	0.0	18.3	3.633223	4530	5616	1248-1248	1.000000	51.0
rps16_p2	rps16	2	TGGCACGACATAAATCCAGT	TCAATTCCCTATACCACTTTGCA	20	23	45.0	39.1	57.2	57.8	0.0	0.0	3.966011	4530	5616	1253-1253	1.000000	48.3
rps16_p3	rps16	3	TGGCACGACATAAATCCAGT	TCCCTATACCACTTTGCAATGAA	20	23	45.0	39.1	57.2	57.8	0.0	18.3	3.966011	4530	5616	1248-1248	1.000000	48.3
rps16_p4	rps16	4	TGGCACGACATAAATCCAGT	TTCCCTATACCACTTTGCAATGA	20	23	45.0	39.1	57.2	57.8	0.0	18.3	3.966011	4530	5616	1249-1249	1.000000	48.3
rps16_p5	rps16	5	TGGCACGACATAAATCCAGT	CCCTATACCACTTTGCAATGA	20	21	45.0	42.9	57.2	55.6	0.0	18.3	4.140464	4530	5616	1247-1247	1.000000	46.9
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5617	7147	1605-1605	1.000000	78.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5617	7147	1616-1616	1.000000	77.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TAAAACCAGAAATGGGGCGT	20	20	45.0	45.0	58.1	57.7	8.1	0.0	0.435397	5617	7147	1665-1665	1.000000	76.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	TAAAACCAGAAATGGGGCGT	20	20	50.0	45.0	58.2	57.7	0.0	0.0	0.500305	5617	7147	1676-1676	1.000000	76.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	5617	7147	1609-1609	1.000000	73.6
petN-trnC-GCA_p1	petN-trnC-GCA	1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	20	19	50.0	52.6	58.2	58.1	0.0	0.0	1.204402	19548	20445	947-948	1.000000	70.5
petN-trnC-GCA_p2	petN-trnC-GCA	2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	20	20	50.0	55.0	58.2	59.1	0.0	4.3	1.260665	19548	20445	949-950	1.000000	70.1
petN-trnC-GCA_p3	petN-trnC-GCA	3	CCACTCCTCCCCCATACTAC	AGTTCAAATCTGGGTGCCG	20	19	60.0	52.6	58.3	58.1	0.0	0.0	1.334774	19548	20445	1003-1004	1.000000	69.5
petN-trnC-GCA_p4	petN-trnC-GCA	4	CCACTCCTCCCCCATACTAC	CCAGTTCAAATCTGGGTGCC	20	20	60.0	55.0	58.3	59.1	0.0	4.3	1.391037	19548	20445	1005-1006	1.000000	69.0
petN-trnC-GCA_p5	petN-trnC-GCA	5	TCCACTCCTCCCCCATACTA	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.4	58.1	0.0	0.0	1.425206	19548	20445	1004-1005	1.000000	68.8
atpI_p1	atpI	1	GCTGAGTTGAGAAAGAGATGGT	AGTTTTCCAACCAAGCGGAT	22	20	45.5	45.0	58.1	58.0	0.0	0.0	2.068060	33052	33795	900-900	1.000000	63.5
atpI_p2	atpI	2	TGCTGAGTTGAGAAAGAGATGG	AGTTTTCCAACCAAGCGGAT	22	20	45.5	45.0	58.1	58.0	0.0	0.0	2.069308	33052	33795	901-901	1.000000	63.5
atpI_p3	atpI	3	ACTTCAAGTTGCTGAGTTGAGA	AGTTTTCCAACCAAGCGGAT	22	20	40.9	45.0	57.9	58.0	0.0	0.0	2.147232	33052	33795	910-910	1.000000	62.9
atpI_p4	atpI	4	ACTTCAAGTTGCTGAGTTGAG	AGTTTTCCAACCAAGCGGAT	21	20	42.9	45.0	56.4	58.0	0.0	0.0	2.604036	33052	33795	910-910	1.000000	59.3
atpI_p5	atpI	5	GCTGAGTTGAGAAAGAGATGG	AGTTTTCCAACCAAGCGGAT	21	20	47.6	45.0	56.3	58.0	0.0	0.0	2.657085	33052	33795	900-900	1.000000	58.8
atpH-atpF_p1	atpH-atpF	1	TGTGGCACTAGCGCTTTTAT	GGATGGCCAGTGAGCTAAAA	20	20	45.0	50.0	57.9	57.9	18.7	13.0	0.236721	34872	35326	542-543	1.000000	78.4
atpH-atpF_p2	atpH-atpF	2	TTGTGGCACTAGCGCTTTTA	GGATGGCCAGTGAGCTAAAA	20	20	45.0	50.0	58.1	57.9	18.7	13.0	0.243211	34872	35326	543-544	1.000000	78.3
atpH-atpF_p3	atpH-atpF	3	TGTGGCACTAGCGCTTTTAT	ATTAAGCCCGAAACTCCCAG	20	20	45.0	50.0	57.9	57.6	18.7	0.0	0.528008	34872	35326	563-564	1.000000	76.1
atpH-atpF_p4	atpH-atpF	4	TTGTGGCACTAGCGCTTTTA	ATTAAGCCCGAAACTCCCAG	20	20	45.0	50.0	58.1	57.6	18.7	0.0	0.534498	34872	35326	564-565	1.000000	76.0
atpH-atpF_p5	atpH-atpF	5	GTGGCACTAGCGCTTTTATT	GGATGGCCAGTGAGCTAAAA	20	20	45.0	50.0	56.8	57.9	18.7	13.0	1.333722	34872	35326	541-542	1.000000	69.6
trnL-UAA_p1	trnL-UAA	1	CGAACTGCTAGACTACGTCG	CTCTTCGCACTCCTTTGTGA	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.229230	49220	49844	844-844	1.000000	78.3
trnL-UAA_p2	trnL-UAA	2	CGAACTGCTAGACTACGTCG	TCTCTTCGCACTCCTTTGTG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.229230	49220	49844	845-845	1.000000	78.3
trnL-UAA_p3	trnL-UAA	3	CGAACTGCTAGACTACGTCG	TGAGTTCTCTTCGCACTCCT	20	20	55.0	50.0	57.9	58.4	0.0	1.5	0.452384	49220	49844	850-850	1.000000	76.5
trnL-UAA_p4	trnL-UAA	4	CGAACTGCTAGACTACGTCG	TTGAGTTCTCTTCGCACTCC	20	20	55.0	50.0	57.9	57.6	0.0	1.5	0.514916	49220	49844	851-851	1.000000	76.0
trnL-UAA_p5	trnL-UAA	5	ACTGCTAGACTACGTCGAGT	CTCTTCGCACTCCTTTGTGA	20	20	50.0	50.0	57.6	57.8	0.0	0.0	0.538222	49220	49844	841-841	1.000000	75.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCCTTCACTAATCGGGGCTA	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.5	57.9	0.0	0.0	0.540775	52518	53261	877-877	1.000000	75.8
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CTGGTCCTTCACTAATCGGG	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	57.4	57.9	0.0	0.0	0.680025	52518	53261	881-881	1.000000	74.7
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCCTTCACTAATCGGGGCTA	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	57.5	58.3	0.0	22.8	0.770156	52518	53261	878-878	1.000000	73.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGGTCCTTCACTAATCGGGG	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	58.8	57.9	0.0	0.0	0.875460	52518	53261	880-880	1.000000	73.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTGGTCCTTCACTAATCGGG	CGGTTCGAATCCGTATAGCC	20	20	55.0	55.0	57.4	58.3	0.0	22.8	0.909407	52518	53261	882-882	1.000000	72.8
atpB-rbcL_p1	atpB-rbcL	1	TTTGATCGATACGCCCTGTG	CCTTGGTTTCATACTCCGGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.103798	56224	57003	953-953	1.000000	79.3
atpB-rbcL_p2	atpB-rbcL	2	TTTGATCGATACGCCCTGTG	CTTGGTTTCATACTCCGGGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.103798	56224	57003	952-952	1.000000	79.3
atpB-rbcL_p3	atpB-rbcL	3	TATCCAGCACGGGTCCAATA	CCTTGGTTTCATACTCCGGG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.242370	56224	57003	974-974	1.000000	78.1
atpB-rbcL_p4	atpB-rbcL	4	TATCCAGCACGGGTCCAATA	CTTGGTTTCATACTCCGGGG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.242370	56224	57003	973-973	1.000000	78.1
atpB-rbcL_p5	atpB-rbcL	5	CCAGCACGGGTCCAATAATT	CTTGGTTTCATACTCCGGGG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.275679	56224	57003	970-970	1.000000	77.9
rbcL_p1	rbcL	1	AGTAGACCCTGTCGTTGTGA	ACTGCATTTCGTAACTGCTT	20	20	50.0	40.0	58.0	56.0	0.0	9.9	2.035646	57004	58437	1565-1565	1.000000	63.8
rbcL_p2	rbcL	2	ATCGAGTAGACCCTGTCGTT	ACTGCATTTCGTAACTGCTT	20	20	50.0	40.0	57.9	56.0	0.0	9.9	2.152540	57004	58437	1569-1569	1.000000	62.8
rbcL_p3	rbcL	3	AGTAGACCCTGTCGTTGTGA	ACTGCATTTCGTAACTGCTTT	20	21	50.0	38.1	58.0	56.7	0.0	9.9	2.272339	57004	58437	1565-1565	1.000000	61.9
rbcL_p4	rbcL	4	TAGACCCTGTCGTTGTGAGA	ACTGCATTTCGTAACTGCTT	20	20	50.0	40.0	57.7	56.0	0.0	9.9	2.308306	57004	58437	1563-1563	1.000000	61.6
rbcL_p5	rbcL	5	GTAGACCCTGTCGTTGTGAG	ACTGCATTTCGTAACTGCTT	20	20	55.0	40.0	57.7	56.0	0.0	9.9	2.376064	57004	58437	1564-1564	1.000000	61.0
rbcL-psaI_p1	rbcL-psaI	1	GCCGCAGCTTGTGAAATATG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.2	58.0	0.0	26.9	0.195661	58438	59729	1431-1441	1.000000	78.8
rbcL-psaI_p2	rbcL-psaI	2	GCCGCAGCTTGTGAAATATG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.2	57.9	0.0	26.8	0.272195	58438	59729	1437-1447	1.000000	78.2
rbcL-psaI_p3	rbcL-psaI	3	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.6	58.0	0.0	26.9	0.380261	58438	59729	1408-1418	1.000000	77.4
rbcL-psaI_p4	rbcL-psaI	4	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.6	58.0	0.0	26.9	0.421933	58438	59729	1413-1423	1.000000	77.0
rbcL-psaI_p5	rbcL-psaI	5	GGCGATCAAATTCGAGTTCG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.6	57.9	0.0	26.8	0.456795	58438	59729	1414-1424	1.000000	76.7
rps18_p1	rps18	1	AGACTACTTCTTCTGGATCCA	GGTCTGGCCCTTTCGAATAA	21	20	42.9	50.0	55.1	57.6	15.3	0.0	4.280034	68244	68735	596-597	1.000000	46.0
rps18_p2	rps18	2	AGACTACTTCTTCTGGATCCA	AGGTCTGGCCCTTTCGAATA	21	20	42.9	50.0	55.1	58.4	15.3	0.0	4.286665	68244	68735	597-598	1.000000	46.0
rps18_p3	rps18	3	AGACTACTTCTTCTGGATCCA	ATAGGTCTGGCCCTTTCGAA	21	20	42.9	50.0	55.1	58.4	15.3	0.0	4.286665	68244	68735	599-600	1.000000	46.0
rps18_p4	rps18	4	AGACTACTTCTTCTGGATCCA	TAGGTCTGGCCCTTTCGAAT	21	20	42.9	50.0	55.1	58.4	15.3	0.0	4.286665	68244	68735	598-599	1.000000	46.0
rps18_p5	rps18	5	TGGACCAAAGAAAGACTACTTCT	GGTCTGGCCCTTTCGAATAA	23	20	39.1	50.0	57.1	57.6	0.0	0.0	4.302292	68244	68735	608-609	1.000000	45.8
petB_p1	petB	1	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATGA	20	21	50.0	47.6	57.6	58.2	0.0	0.0	1.620707	73853	75316	1571-1572	1.000000	67.1
petB_p2	petB	2	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATG	20	20	50.0	50.0	57.6	56.7	0.0	0.0	1.668360	73853	75316	1571-1572	1.000000	66.7
petB_p3	petB	3	GGTAGTTCGACCGTGGAATT	TGAAATACCACAACCTACCCG	20	21	50.0	47.6	57.6	57.7	0.0	0.0	1.712867	73853	75316	1577-1578	1.000000	66.3
petB_p4	petB	4	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATGA	20	21	50.0	47.6	57.4	58.2	0.0	0.0	1.894865	73853	75316	1576-1577	1.000000	64.9
petB_p5	petB	5	CATTCTGGTAGTTCGACCGT	ACCACAACCTACCCGATATGA	20	21	50.0	47.6	57.4	58.2	0.0	0.0	1.894865	73853	75316	1577-1578	1.000000	64.9
rpl22_p1	rpl22	1	GTGCGAACCAAAAGGAATGG	CAAATTGGGGGAATTCGTGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.073325	82789	83238	723-723	1.000000	79.9
rpl22_p2	rpl22	2	GTGCGAACCAAAAGGAATGG	GCAAATTGGGGGAATTCGTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.073325	82789	83238	724-724	1.000000	79.9
rpl22_p3	rpl22	3	CAGAACTTGAGCCTGAGTCC	CAAATTGGGGGAATTCGTGC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.100604	82789	83238	891-891	1.000000	79.6
rpl22_p4	rpl22	4	CAGAACTTGAGCCTGAGTCC	GCAAATTGGGGGAATTCGTG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.100604	82789	83238	892-892	1.000000	79.6
rpl22_p5	rpl22	5	GTGCGAACCAAAAGGAATGG	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.088749	82789	83238	1264-1264	1.000000	79.5
rpl22-rps19_p1	rpl22-rps19	1	CGAGTTTCCTTAGCCCACTC	CAAATTGGGGGAATTCGTGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.020292	83239	83302	213-213	1.000000	81.3
rpl22-rps19_p2	rpl22-rps19	2	CGAGTTTCCTTAGCCCACTC	GCAAATTGGGGGAATTCGTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.020292	83239	83302	214-214	1.000000	81.3
rpl22-rps19_p3	rpl22-rps19	3	TCCTTGCGAGTTTCCTTAGC	CAAATTGGGGGAATTCGTGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.103514	83239	83302	219-219	1.000000	80.6
rpl22-rps19_p4	rpl22-rps19	4	TCCTTGCGAGTTTCCTTAGC	GCAAATTGGGGGAATTCGTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.103514	83239	83302	220-220	1.000000	80.6
rpl22-rps19_p5	rpl22-rps19	5	CGAGTTTCCTTAGCCCACTC	ATGGTAGGTCGCAAATTGGG	20	20	55.0	50.0	58.0	58.2	0.0	3.4	0.248833	83239	83302	224-224	1.000000	79.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGGGTTTTTCTGGGCAACA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	108472	109174	775-775	1.000000	78.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGTTTTTCTGGGCAACAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	108472	109174	774-774	1.000000	78.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGATCATTTTCCAGCGGCA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.2	57.9	1.4	0.0	0.286165	108472	109174	812-812	1.000000	78.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAGGGTTTTTCTGGGCAACA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.312231	108472	109174	768-768	1.000000	78.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGTTTTTCTGGGCAACAA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.312231	108472	109174	767-767	1.000000	78.0
ccsA_p1	ccsA	1	AGTGGCGGCATTCTCAAAAA	TTTGAGAAGGCGTTCAAGGG	20	20	45.0	50.0	58.7	58.4	17.2	20.4	1.068094	109332	110309	1144-1144	1.000000	71.5
ccsA_p2	ccsA	2	AGTGGCGGCATTCTCAAAAA	GGCGTTCAAGGGTTCTCAAA	20	20	45.0	50.0	58.7	58.7	17.2	0.0	1.357967	109332	110309	1136-1136	1.000000	69.2
ccsA_p3	ccsA	3	AGTGGCGGCATTCTCAAAA	TTTGAGAAGGCGTTCAAGGG	19	20	47.4	50.0	58.0	58.4	17.2	20.4	1.430320	109332	110309	1144-1144	1.000000	68.6
ccsA_p4	ccsA	4	GAGTGGCGGCATTCTCAAAA	TTTGAGAAGGCGTTCAAGGG	20	20	50.0	50.0	59.1	58.4	24.4	20.4	1.514278	109332	110309	1145-1145	1.000000	68.0
ccsA_p5	ccsA	5	AGTGGCGGCATTCTCAAAAA	GAAGGCGTTCAAGGGTTCTC	20	20	45.0	55.0	58.7	58.8	17.2	14.1	1.515113	109332	110309	1139-1139	1.000000	67.9