Hotspot

rpoA

LSC · 77492-78502 · 1011 bp

Back to Achyranthes

Pi 0.00198

MarkerSeek score 60.00

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1011
variable_sites: 3
pi: 0.00198
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	AGGGAATAGTCGCTTCAAAGT	CCACATAATGGCTGTAGACCT	42.9	47.6	57.0	57.2	1183 bp	1.0	49.6
rpoA_p2	GGGAATAGTCGCTTCAAAGTG	CCACATAATGGCTGTAGACCT	47.6	47.6	57.0	57.2	1182 bp	1.0	49.3
rpoA_p3	TCGCTTCAAAGTGAATTTTCCC	CCACATAATGGCTGTAGACCT	40.9	47.6	57.8	57.2	1174 bp	1.0	47.5
rpoA_p4	CGCTTCAAAGTGAATTTTCCCT	CCACATAATGGCTGTAGACCT	40.9	47.6	57.8	57.2	1173 bp	1.0	47.5
rpoA_p5	AGGGAATAGTCGCTTCAAAGT	ACATAATGGCTGTAGACCTCC	42.9	47.6	57.0	56.9	1181 bp	1.0	47.3