Hotspot

psbD

LSC · 32846-33907 · 1062 bp

Back to Achyranthes

Pi 0.00126

MarkerSeek score 59.60

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.00126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	TGCTCGGAAATGGTTGAAGT	GATAAGTCGAGCATTCCCGG	45.0	55.0	57.7	58.2	1178 bp	1.0	76.2
psbD_p2	CCAAAAATGCTATGGGGTGC	GATAAGTCGAGCATTCCCGG	50.0	55.0	57.7	58.2	1195 bp	1.0	75.9
psbD_p3	CTATGGGGTGCTCGGAAATG	GATAAGTCGAGCATTCCCGG	55.0	55.0	58.4	58.2	1186 bp	1.0	75.2
psbD_p4	TGCTCGGAAATGGTTGAAGT	TGATAAGTCGAGCATTCCCG	45.0	50.0	57.7	57.5	1179 bp	1.0	73.9
psbD_p5	AAAATGCTATGGGGTGCTCG	GATAAGTCGAGCATTCCCGG	50.0	55.0	58.6	58.2	1192 bp	1.0	73.6