Hotspot

psaA

LSC · 39713-41965 · 2253 bp

Back to Achyranthes

Pi 0.000888

MarkerSeek score 57.70

Primer available yes

Label psaA

Feature Metadata

label_name: psaA
feature_type: gene
region: LSC
length_bp: 2253
variable_sites: 3
pi: 0.000888
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA_p1	AGCTAAGCCTTGGCTAAACC	TGTCTTGTCCGGAAAGAGGA	50.0	50.0	57.9	58.3	2344 bp	1.0	76.8
psaA_p2	GCTAAGCCTTGGCTAAACCT	TGTCTTGTCCGGAAAGAGGA	50.0	50.0	57.9	58.3	2343 bp	1.0	76.8
psaA_p3	AATACGACGAGTAGTGGGGT	TGTCTTGTCCGGAAAGAGGA	50.0	50.0	57.9	58.3	2368 bp	1.0	76.7
psaA_p4	GTAGTGGGGTCCTGAGCTAA	TGTCTTGTCCGGAAAGAGGA	55.0	50.0	58.1	58.3	2358 bp	1.0	76.7
psaA_p5	TAGTGGGGTCCTGAGCTAAG	TGTCTTGTCCGGAAAGAGGA	55.0	50.0	57.8	58.3	2357 bp	1.0	76.5