Hotspot

ndhC

LSC · 50230-50592 · 363 bp

Back to Achyranthes

Pi 0.00184

MarkerSeek score 29.10

Primer available yes

Label ndhC

Feature Metadata

label_name: ndhC
feature_type: gene
region: LSC
length_bp: 363
variable_sites: 1
pi: 0.00184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC_p1	ATTGTCGGAATATTCGGGGA	TCCACATTCATAAGGAGTTCGT	45.0	40.9	56.4	57.2	407 bp	1.0	44.8
ndhC_p2	ATTGTCGGAATATTCGGGGA	AGGTGGTTCACTCAAAATGA	45.0	40.0	56.4	54.8	498 bp	1.0	43.9
ndhC_p3	ATTGTCGGAATATTCGGGGA	CCACATTCATAAGGAGTTCGTC	45.0	45.5	56.4	56.9	406 bp	1.0	42.3
ndhC_p4	ATTGTCGGAATATTCGGGGA	TTCCACATTCATAAGGAGTTCGT	45.0	39.1	56.4	57.8	408 bp	1.0	42.0
ndhC_p5	ATTGTCGGAATATTCGGGGA	CCACATTCATAAGGAGTTCGTCT	45.0	43.5	56.4	58.2	406 bp	1.0	41.4