Hotspot

matK

LSC · 2086-3597 · 1512 bp

Back to Achyranthes

Pi 0.00176

MarkerSeek score 58.20

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 4
pi: 0.00176
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TACTCAATGGCTTCAACCCC	CCTTGTTTTGACTGTATCGCA	50.0	42.9	57.5	56.8	1708-1710 bp	1.0	58.8
matK_p2	TACTCAATGGCTTCAACCCC	ACCTTGTTTTGACTGTATCGC	50.0	42.9	57.5	56.8	1709-1711 bp	1.0	58.8
matK_p3	TACTCAATGGCTTCAACCCC	CCTTGTTTTGACTGTATCGCAC	50.0	45.5	57.5	58.2	1708-1710 bp	1.0	58.6
matK_p4	CTCAATGGCTTCAACCCCTA	CCTTGTTTTGACTGTATCGCA	50.0	42.9	57.2	56.8	1706-1708 bp	1.0	56.4
matK_p5	CTCAATGGCTTCAACCCCTA	ACCTTGTTTTGACTGTATCGC	50.0	42.9	57.2	56.8	1707-1709 bp	1.0	56.4