Hotspot

ndhI-ndhA

SSC · 118305-119883 · 1579 bp

Back to Achyranthes

Pi 0.000846

MarkerSeek score 49.20

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 1579
variable_sites: 2
pi: 0.000846
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TTACGGGTAAACGGTTAGCG	ATCTCTACGTGTGACTCGTT	50.0	45.0	58.0	56.0	1714-1716 bp	1.0	64.6
ndhI-ndhA_p2	GTAAACGGTTAGCGTGGGAT	ATCTCTACGTGTGACTCGTT	50.0	45.0	58.0	56.0	1708-1710 bp	1.0	64.6
ndhI-ndhA_p3	GCGTGGGATAGCGTAATCAT	ATCTCTACGTGTGACTCGTT	50.0	45.0	57.9	56.0	1697-1699 bp	1.0	64.0
ndhI-ndhA_p4	AAACGGTTAGCGTGGGATAG	ATCTCTACGTGTGACTCGTT	50.0	45.0	57.7	56.0	1706-1708 bp	1.0	62.2
ndhI-ndhA_p5	TTGACCAATGTACCTTGCCG	ATCTCTACGTGTGACTCGTT	50.0	45.0	58.5	56.0	1671-1673 bp	1.0	60.9