Hotspot

trnN-ndhF

IRb · 107307-109072 · 1766 bp

Back to Achyranthes

Pi 0.000755

MarkerSeek score 44.30

Primer available yes

Label trnN-ndhF

Feature Metadata

label_name: trnN-ndhF
feature_type: igs
region: IRb
length_bp: 1766
variable_sites: 2
pi: 0.000755
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-ndhF_p1	GCAACGCAACCAGCATATTT	AGGGGGTCGAATCTCTTCAT	45.0	50.0	58.0	57.8	2342 bp	1.0	78.7
trnN-ndhF_p2	GGCAGAATAGCAGAGCAAGT	AGGGGGTCGAATCTCTTCAT	50.0	50.0	58.0	57.8	2281 bp	1.0	78.4
trnN-ndhF_p3	GCGAGCCCCTTCATTCTTTA	AGGGGGTCGAATCTCTTCAT	50.0	50.0	58.0	57.8	1958 bp	1.0	78.4
trnN-ndhF_p4	CGGGATAGCGAAATAGCGAA	AGGGGGTCGAATCTCTTCAT	50.0	50.0	57.9	57.8	2473 bp	1.0	78.2
trnN-ndhF_p5	GAAGGACCTTTCCCCTTTGG	AGGGGGTCGAATCTCTTCAT	55.0	50.0	58.1	57.8	2511 bp	1.0	78.1