Hotspot

trnT-atpE

LSC · 51929-52104 · 176 bp

Back to Achyranthes

Pi 0.00758
MarkerSeek score 44.80
Primer available yes
Label trnT-atpE

Feature Metadata

label_name
trnT-atpE
feature_type
igs
region
LSC
length_bp
176
variable_sites
2
pi
0.00758
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-atpE_p1 TATTGCTTTCATACGGCGGG ACACTAGTAGAGGCTGGCAA 50.0 50.0 58.4 58.1 250 bp 1.0 76.9
trnT-atpE_p2 TATTGCTTTCATACGGCGGG CGAGCGAGGACACTAGTAGA 50.0 55.0 58.4 58.1 259 bp 1.0 76.8
trnT-atpE_p3 TATTGCTTTCATACGGCGGG GCTAACTTGAGGAAAGCGGA 50.0 50.0 58.4 57.9 319 bp 1.0 76.5
trnT-atpE_p4 TATTGCTTTCATACGGCGGG AGCTAACTTGAGGAAAGCGG 50.0 50.0 58.4 57.9 320 bp 1.0 76.4
trnT-atpE_p5 TATTGCTTTCATACGGCGGG AACTTGAGGAAAGCGGAAGG 50.0 50.0 58.4 58.1 316 bp 1.0 76.4