Hotspot

trnT-trnL

LSC · 46651-47322 · 672 bp

Back to Achyranthes

Pi 0.00298

MarkerSeek score 60.80

Primer available yes

Label trnT-trnL

Feature Metadata

label_name: trnT-trnL
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 3
pi: 0.00298
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnL_p1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	55.0	50.0	57.9	58.1	744-745 bp	1.0	78.9
trnT-trnL_p2	GGAATCGAACCGATGACCAT	AGCCAATTAAGTCCGTAGCG	50.0	50.0	57.8	58.1	776-777 bp	1.0	78.0
trnT-trnL_p3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	732-733 bp	1.0	77.4
trnT-trnL_p4	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	55.0	50.0	57.9	58.4	743-744 bp	1.0	76.6
trnT-trnL_p5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	764-765 bp	1.0	76.5