Hotspot

psaA-ycf3

LSC · 41966-42772 · 807 bp

Back to Achyranthes

Pi 0

MarkerSeek score 59.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 807
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTACTTCTGGTTCCGGCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.5	58.0	937-938 bp	1.0	76.0
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	871-872 bp	1.0	75.3
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.5	58.1	939-940 bp	1.0	75.2
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.5	58.3	933-934 bp	1.0	74.2
psaA-ycf3_p5	TTTTACTTCTGGTTCCGGCG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.5	58.3	935-936 bp	1.0	74.2