Hotspot

psbZ-trnM

LSC · 36164-36828 · 665 bp

Back to Achyranthes

Pi 0.00201

MarkerSeek score 64.80

Primer available yes

Label psbZ-trnM

Feature Metadata

label_name: psbZ-trnM
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 2
pi: 0.00201
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnM_p1	TGTATTTGCTTCTCCCGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	787-815 bp	1.0	82.6
psbZ-trnM_p2	TGTATTTGCTTCTCCCGACG	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	781-809 bp	1.0	82.1
psbZ-trnM_p3	CGACGGTTGGTCAAGTAACA	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	772-800 bp	1.0	81.6
psbZ-trnM_p4	TGTATTTGCTTCTCCCGACG	TCACGGGTTCAAATCCTGTC	50.0	50.0	58.0	57.8	780-808 bp	1.0	81.5
psbZ-trnM_p5	TGTATTTGCTTCTCCCGACG	CCATTCGTTCTCAGCCCTTT	50.0	50.0	58.0	58.2	854-882 bp	1.0	81.3