Hotspot

psbM-trnD

LSC · 29258-30310 · 1053 bp

Back to Achyranthes

Pi 0.0019

MarkerSeek score 47.50

Primer available yes

Label psbM-trnD

Feature Metadata

label_name: psbM-trnD
feature_type: igs
region: LSC
length_bp: 1053
variable_sites: 3
pi: 0.0019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_p1	TCATTTTGGCTGACCGTTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	56.4	58.6	1217-1220 bp	1.0	63.3
psbM-trnD_p2	AATCATTTTGGCTGACCGTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	56.2	58.6	1219-1222 bp	1.0	61.5
psbM-trnD_p3	ATCATTTTGGCTGACCGTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	56.2	58.6	1218-1221 bp	1.0	61.5
psbM-trnD_p4	TCATTTTGGCTGACCGTTTTT	TCAATTGGTTAGAGCACCGC	38.1	50.0	57.2	58.6	1217-1220 bp	1.0	61.3
psbM-trnD_p5	TTTGGCTGACCGTTTTTACA	TCAATTGGTTAGAGCACCGC	40.0	50.0	56.1	58.6	1213-1216 bp	1.0	60.5