Hotspot

rpoB-trnC

LSC · 26474-27307 · 834 bp

Back to Achyranthes

Pi 0.0024

MarkerSeek score 60.30

Primer available yes

Label rpoB-trnC

Feature Metadata

label_name: rpoB-trnC
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 3
pi: 0.0024
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC_p1	AAGCCCCGATCAATGAATCT	CGGATTTGAACTGGGGAACA	45.0	50.0	57.0	58.1	987 bp	1.0	71.2
rpoB-trnC_p2	GTTCTTCTCTTAAGCCCCGA	CGGATTTGAACTGGGGAACA	50.0	50.0	56.9	58.1	998 bp	1.0	71.1
rpoB-trnC_p3	AGTTCTTCTCTTAAGCCCCG	CGGATTTGAACTGGGGAACA	50.0	50.0	56.9	58.1	999 bp	1.0	71.0
rpoB-trnC_p4	CTTCATTTCCATCCCGTAGCA	CGGATTTGAACTGGGGAACA	47.6	50.0	58.1	58.1	917 bp	1.0	70.8
rpoB-trnC_p5	TTCATTTCCATCCCGTAGCA	CGGATTTGAACTGGGGAACA	45.0	50.0	56.9	58.1	916 bp	1.0	70.8