Hotspot

petD

LSC · 74007-75239 · 1233 bp

Back to Achnatherum

Pi 0.00216

MarkerSeek score 60.10

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1233
variable_sites: 4
pi: 0.00216
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACTTCTCTTCAACTCCGAAGT	CACGGTACTTCACGGTTGAA	42.9	50.0	56.6	58.1	1351-1356 bp	0.666667	46.6
petD_p2	ACGAAAGAAAATAAGGCGGA	CACGGTACTTCACGGTTGAA	40.0	50.0	55.4	58.1	1295 bp	0.666667	44.7
petD_p3	ACTTCTCTTCAACTCCGAAGT	CCTATGCACGGTACTTCACG	42.9	55.0	56.6	58.4	1357-1362 bp	0.666667	44.2
petD_p4	ACTTCTCTTCAACTCCGAAGT	CTATGCACGGTACTTCACGG	42.9	55.0	56.6	58.4	1356-1361 bp	0.666667	44.2
petD_p5	TGGATACTTCTCTTCAACTCCG	CACGGTACTTCACGGTTGAA	45.5	50.0	57.3	58.1	1356-1361 bp	0.666667	43.9