Hotspot

ndhH

SSC · 115048-116229 · 1182 bp

Back to Achnatherum

Pi 0.00733

MarkerSeek score 53.10

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 13
pi: 0.00733
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TGATAGTTTCTACCTCTACCCT	TGCATTTTGATGAGCCTCGT	40.9	45.0	54.8	58.2	1296 bp	1.0	40.8
ndhH_p2	TCCGATTTCGAAAAAGAATTGA	TGCATTTTGATGAGCCTCGT	31.8	45.0	54.7	58.2	1315 bp	1.0	40.8
ndhH_p3	TTGATAGTTTCTACCTCTACCCT	TGCATTTTGATGAGCCTCGT	39.1	45.0	55.6	58.2	1297 bp	1.0	40.8
ndhH_p4	AGTTTCTACCTCTACCCTATCT	TGCATTTTGATGAGCCTCGT	40.9	45.0	54.5	58.2	1292 bp	1.0	40.8
ndhH_p5	TTCCGATTTCGAAAAAGAATTGA	TGCATTTTGATGAGCCTCGT	30.4	45.0	55.4	58.2	1316 bp	1.0	40.8