Hotspot

psaC-ndhE

SSC · 110537-111016 · 480 bp

Back to Achnatherum

Pi 0.00694

MarkerSeek score 60.70

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 480
variable_sites: 5
pi: 0.00694
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GCTTTACATCCATCCCAGGG	AGCCGCTATTGGACTATCCA	55.0	50.0	58.0	58.3	675-680 bp	1.0	78.2
psaC-ndhE_p2	GGCTTTACATCCATCCCAGG	AGCCGCTATTGGACTATCCA	55.0	50.0	58.0	58.3	676-681 bp	1.0	78.2
psaC-ndhE_p3	GTTGGACAAGCTCGTACACA	AGCCGCTATTGGACTATCCA	50.0	50.0	58.1	58.3	636-641 bp	1.0	77.2
psaC-ndhE_p4	TGTTGGACAAGCTCGTACAC	AGCCGCTATTGGACTATCCA	50.0	50.0	58.1	58.3	637-642 bp	1.0	77.2
psaC-ndhE_p5	CTGTTGGACAAGCTCGTACA	AGCCGCTATTGGACTATCCA	50.0	50.0	57.9	58.3	638-643 bp	1.0	77.2