Hotspot

rpl32-trnL-UAG

SSC · 106637-107358 · 722 bp

Back to Achnatherum

Pi 0.014
MarkerSeek score 61.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
722
variable_sites
15
pi
0.014
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 763-794 bp 1.0 83.3
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 762-793 bp 1.0 83.3
rpl32-trnL-UAG_p3 AAGGGTTTTTCTGGGCAACA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 756-787 bp 1.0 82.9
rpl32-trnL-UAG_p4 AGGGTTTTTCTGGGCAACAA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 755-786 bp 1.0 82.9
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.1 57.8 760-791 bp 1.0 82.8