Hotspot

ndhH-ndhF

SSC · 103298-103335 · 38 bp

Back to Achnatherum

Pi 0.0351

MarkerSeek score 61.00

Primer available yes

Label ndhH-ndhF

Feature Metadata

label_name: ndhH-ndhF
feature_type: igs
region: SSC
length_bp: 38
variable_sites: 2
pi: 0.0351
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-ndhF_p1	AACACCCATCAATGCATGGT	ATGGAGTGGGTCTTGCTAGT	45.0	50.0	58.1	58.0	246-350 bp	1.0	80.3
ndhH-ndhF_p2	ACCCATCAATGCATGGTGTT	ATGGAGTGGGTCTTGCTAGT	45.0	50.0	58.1	58.0	243-347 bp	1.0	80.3
ndhH-ndhF_p3	CTTTTGGGAAAACGTCGACG	ATGGAGTGGGTCTTGCTAGT	50.0	50.0	58.0	58.0	534-638 bp	1.0	80.2
ndhH-ndhF_p4	CTTTTGGGAAAACGTCGACG	TAGCCTATCCGCATGAAACG	50.0	50.0	58.0	57.9	1035-1139 bp	1.0	79.9
ndhH-ndhF_p5	ACCCATCAATGCATGGTGTT	TAGCCTATCCGCATGAAACG	45.0	50.0	58.1	57.9	744-848 bp	1.0	79.8