Hotspot

trnN-GUU-rps15

IRb · 101225-102755 · 1531 bp

Back to Achnatherum

Pi 0.00218

MarkerSeek score 60.10

Primer available yes

Label trnN-GUU-rps15

Feature Metadata

label_name: trnN-GUU-rps15
feature_type: igs
region: IRb
length_bp: 1531
variable_sites: 5
pi: 0.00218
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rps15_p1	AGGATTCGAACCTACGACCA	CAGAACCCCTGTTTTCTTCT	50.0	45.0	58.2	55.2	1667 bp	1.0	56.3
trnN-GUU-rps15_p2	GGATTCGAACCTACGACCAG	CAGAACCCCTGTTTTCTTCT	55.0	45.0	57.8	55.2	1666 bp	1.0	55.9
trnN-GUU-rps15_p3	AGGATTCGAACCTACGACCA	ACTTGAAATTCAACAGAACCCC	50.0	40.9	58.2	57.1	1680 bp	1.0	55.5
trnN-GUU-rps15_p4	GGATTCGAACCTACGACCAG	ACTTGAAATTCAACAGAACCCC	55.0	40.9	57.8	57.1	1679 bp	1.0	55.1
trnN-GUU-rps15_p5	ACCACTGAGCTACTGAGGAA	CAGAACCCCTGTTTTCTTCT	50.0	45.0	57.7	55.2	1624 bp	1.0	55.1