Hotspot

rpl20-rps12

LSC · 67805-68501 · 697 bp

Back to Achnatherum

Pi 0.00862

MarkerSeek score 53.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 697
variable_sites: 9
pi: 0.00862
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CGTCGTCTCCGGGCTATATA	GAGGAACATGTGCTAGGGTG	55.0	55.0	58.3	58.0	760-761 bp	1.0	78.7
rpl20-rps12_p2	GGGCTATATATCCTCGCGGA	GAGGAACATGTGCTAGGGTG	55.0	55.0	58.3	58.0	750-751 bp	1.0	78.3
rpl20-rps12_p3	TCCGGGCTATATATCCTCGC	GAGGAACATGTGCTAGGGTG	55.0	55.0	58.3	58.0	753-754 bp	1.0	78.3
rpl20-rps12_p4	CGTCGTCTCCGGGCTATATA	GTCGAGGAACATGTGCTAGG	55.0	55.0	58.3	58.1	763-764 bp	1.0	78.3
rpl20-rps12_p5	GGGCTATATATCCTCGCGGA	GTCGAGGAACATGTGCTAGG	55.0	55.0	58.3	58.1	753-754 bp	1.0	78.0