Hotspot

rpl33-rps18

LSC · 66508-66779 · 272 bp

Back to Achnatherum

Pi 0
MarkerSeek score 41.50
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
272
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 ACGACTCATCACGAAATAAAGA TGGATTTACGAAAGGGTTGC 36.4 45.0 55.6 56.3 349-354 bp 1.0 41.1
rpl33-rps18_p2 ACGACTCATCACGAAATAAAGA TTGGATTTACGAAAGGGTTGC 36.4 42.9 55.6 57.1 350-355 bp 1.0 41.1
rpl33-rps18_p3 ACGACTCATCACGAAATAAAGAA TGGATTTACGAAAGGGTTGC 34.8 45.0 56.3 56.3 349-354 bp 1.0 41.1
rpl33-rps18_p4 ACGACTCATCACGAAATAAAG TGGATTTACGAAAGGGTTGC 38.1 45.0 54.1 56.3 349-354 bp 1.0 41.1
rpl33-rps18_p5 ACGACTCATCACGAAATAAAGAA TTGGATTTACGAAAGGGTTGC 34.8 42.9 56.3 57.1 350-355 bp 1.0 41.1