Hotspot

ndhC-trnV-UAC

LSC · 50735-51609 · 875 bp

Back to Achnatherum

Pi 0.00343

MarkerSeek score 63.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 4
pi: 0.00343
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	1001-1021 bp	1.0	80.8
ndhC-trnV-UAC_p2	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.0	58.3	1002-1022 bp	1.0	78.9
ndhC-trnV-UAC_p3	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	1000-1020 bp	1.0	78.3
ndhC-trnV-UAC_p4	CACTAACCGGGGCTAAAAGT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	1003-1023 bp	1.0	77.2
ndhC-trnV-UAC_p5	TAACCGGGGCTAAAAGTCCT	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.3	58.3	1001-1021 bp	1.0	76.5