Hotspot

atpI-atpH

LSC · 32365-32895 · 531 bp

Back to Achnatherum

Pi 0.00628

MarkerSeek score 61.70

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 531
variable_sites: 5
pi: 0.00628
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TTTTGCAACATTAGCCGCAG	GCAATAACAGAAGCAGCAGC	45.0	50.0	57.9	58.1	621-628 bp	1.0	79.6
atpI-atpH_p2	TTTTGCAACATTAGCCGCAG	GCAGCAATAACAGAAGCAGC	45.0	50.0	57.9	58.1	624-631 bp	1.0	79.6
atpI-atpH_p3	TTTTGCAACATTAGCCGCAG	AAGCAGCAGCAATTAGTGGA	45.0	45.0	57.9	57.8	611-618 bp	1.0	78.8
atpI-atpH_p4	TTTTGCAACATTAGCCGCAG	GCCAATCCAGCAGCAATAAC	45.0	50.0	57.9	57.8	633-640 bp	1.0	78.7
atpI-atpH_p5	TTTTGCAACATTAGCCGCAG	CCTTCTACAGCTTGTCCTGC	45.0	55.0	57.9	58.3	699-706 bp	1.0	78.1