Hotspot

trnG-UCC-trnT-GGU

LSC · 13561-15204 · 1644 bp

Back to Achnatherum

Pi 0.00617

MarkerSeek score 52.50

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1644
variable_sites: 15
pi: 0.00617
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	TGACTTACGTCTTACCACGC	38.1	50.0	55.5	57.7	1781-1796 bp	1.0	50.7
trnG-UCC-trnT-GGU_p2	AGAACGAATCACACTTTTACCAC	TGACTTACGTCTTACCACGC	39.1	50.0	57.3	57.7	1784-1799 bp	1.0	49.7
trnG-UCC-trnT-GGU_p3	GAACGAATCACACTTTTACCACT	TGACTTACGTCTTACCACGC	39.1	50.0	57.3	57.7	1783-1798 bp	1.0	49.7
trnG-UCC-trnT-GGU_p4	AACGAATCACACTTTTACCACT	TGACTTACGTCTTACCACGC	36.4	50.0	56.2	57.7	1782-1797 bp	1.0	48.8
trnG-UCC-trnT-GGU_p5	ACGAATCACACTTTTACCACT	GACTTACGTCTTACCACGCT	38.1	50.0	55.5	57.4	1780-1795 bp	1.0	48.4