Hotspot

trnG-GCC-trnM-CAU

LSC · 12152-12651 · 500 bp

Back to Achnatherum

Pi 0.00418

MarkerSeek score 60.30

Primer available yes

Label trnG-GCC-trnM-CAU

Feature Metadata

label_name: trnG-GCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 500
variable_sites: 3
pi: 0.00418
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnM-CAU_p1	GTCGACCCAAGCGGATATAC	GTTTCCAGTACTCCAGAGCG	55.0	55.0	57.9	58.0	682-703 bp	1.0	82.3
trnG-GCC-trnM-CAU_p2	GCGAGCGTAGTTCAATGGTA	GTTTCCAGTACTCCAGAGCG	50.0	55.0	58.1	58.0	642-663 bp	1.0	82.3
trnG-GCC-trnM-CAU_p3	TAGCGAGCGTAGTTCAATGG	GTTTCCAGTACTCCAGAGCG	50.0	55.0	57.8	58.0	644-665 bp	1.0	81.3
trnG-GCC-trnM-CAU_p4	TATCCTGTAGCGAGCGTAGT	GTTTCCAGTACTCCAGAGCG	50.0	55.0	57.7	58.0	651-672 bp	1.0	80.9
trnG-GCC-trnM-CAU_p5	CTGTAGCGAGCGTAGTTCAA	GTTTCCAGTACTCCAGAGCG	50.0	55.0	57.7	58.0	647-668 bp	1.0	80.9