Hotspot

ccsA

SSC · 113884-114855 · 972 bp

Back to Achimenes

Pi 0.0072

MarkerSeek score 48.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 7
pi: 0.0072
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GAGTTCAAGACAAGCCGCTA	TCAGTAGCTTTTCGATGGCC	50.0	50.0	57.9	58.0	1754-1759 bp	1.0	79.9
ccsA_p2	GAGTTCAAGACAAGCCGCTA	CAGTAGCTTTTCGATGGCCT	50.0	50.0	57.9	58.0	1753-1758 bp	1.0	79.9
ccsA_p3	GAGTTCAAGACAAGCCGCTA	TGGTGCTGCACTCTTTTTCT	50.0	45.0	57.9	57.9	1864-1869 bp	1.0	79.6
ccsA_p4	TAGACACGCTGCTCTTAGGA	TCAGTAGCTTTTCGATGGCC	50.0	50.0	57.9	58.0	1722-1727 bp	1.0	79.6
ccsA_p5	ACAAGCCGCTATGGTGAAAT	TCAGTAGCTTTTCGATGGCC	45.0	50.0	57.9	58.0	1745-1750 bp	1.0	79.6