Hotspot

psbH-petB

LSC · 75481-76302 · 822 bp

Back to Achimenes

Pi 0.0073

MarkerSeek score 62.90

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 822
variable_sites: 6
pi: 0.0073
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	TCCTTTGATGGGTGTTGCAA	CGAGACGTTCTTCGAACCAA	45.0	50.0	57.9	58.2	974 bp	1.0	78.0
psbH-petB_p2	TCCTTTGATGGGTGTTGCAA	ATATCATCCGCAATCGCCTG	45.0	50.0	57.9	58.3	999 bp	1.0	77.7
psbH-petB_p3	AACGACTCCTTTGATGGGTG	CGAGACGTTCTTCGAACCAA	50.0	50.0	57.8	58.2	980 bp	1.0	77.1
psbH-petB_p4	CGACTCCTTTGATGGGTGTT	CGAGACGTTCTTCGAACCAA	50.0	50.0	57.8	58.2	978 bp	1.0	77.1
psbH-petB_p5	CGACTCCTTTGATGGGTGTT	ATATCATCCGCAATCGCCTG	50.0	50.0	57.8	58.3	1003 bp	1.0	76.8