Hotspot

psbT-psbN

LSC · 74957-75021 · 65 bp

Back to Achimenes

Pi 0.0308

MarkerSeek score 70.20

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 2
pi: 0.0308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	GCGAACCACCTAAAGTTCCA	TCGAGGAACATGGGGACTAG	50.0	55.0	58.1	58.2	117 bp	1.0	78.7
psbT-psbN_p2	GCGAACCACCTAAAGTTCCA	TTCGAGGAACATGGGGACTA	50.0	50.0	58.1	57.8	118 bp	1.0	78.5
psbT-psbN_p3	TCGCGAACCACCTAAAGTTC	TCGAGGAACATGGGGACTAG	50.0	55.0	58.2	58.2	119 bp	1.0	77.9
psbT-psbN_p4	GCGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	50.0	55.0	58.1	57.7	172 bp	1.0	77.8
psbT-psbN_p5	GCGAACCACCTAAAGTTCCA	CATTCGAGGAACATGGGGAC	50.0	55.0	58.1	58.3	120 bp	1.0	77.8