Hotspot

atpB-rbcL

LSC · 54898-55676 · 779 bp

Back to Achimenes

Pi 0.00385

MarkerSeek score 61.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 3
pi: 0.00385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	869 bp	1.0	77.2
atpB-rbcL_p2	ATGATTTGGACGATACGCCC	AACACCCGCTTTGAATCCAA	50.0	45.0	58.1	58.3	910 bp	1.0	76.9
atpB-rbcL_p3	CCGGACCTATGATTTGGACG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	918 bp	1.0	76.8
atpB-rbcL_p4	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.8	58.3	940 bp	1.0	76.6
atpB-rbcL_p5	GGAAACCCCAGAACCAGAAG	TAACACCCGCTTTGAATCCA	55.0	45.0	58.1	57.4	870 bp	1.0	75.0