Hotspot

ndhC-trnM-CAU

LSC · 51002-52719 · 1718 bp

Back to Achimenes

Pi 0.00936

MarkerSeek score 60.40

Primer available yes

Label ndhC-trnM-CAU

Feature Metadata

label_name: ndhC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1718
variable_sites: 16
pi: 0.00936
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAU_p1	GGGCTAAGATCCCCGAAATG	CCCGCCGTATGAAAGCAATA	55.0	50.0	58.1	58.4	1850-1859 bp	1.0	77.0
ndhC-trnM-CAU_p2	GGGCTAAGATCCCCGAAATG	CCGCCGTATGAAAGCAATAC	55.0	50.0	58.1	57.3	1849-1858 bp	1.0	75.0
ndhC-trnM-CAU_p3	TTCTCCGGCCCCTTTCTAAT	CCCGCCGTATGAAAGCAATA	50.0	50.0	58.4	58.4	1872-1881 bp	1.0	74.5
ndhC-trnM-CAU_p4	GGGCTAAGATCCCCGAAATG	TTTGAACCAATGACTCCCGC	55.0	50.0	58.1	58.8	1865-1874 bp	1.0	74.2
ndhC-trnM-CAU_p5	TTTCTCCGGCCCCTTTCTAA	CCCGCCGTATGAAAGCAATA	50.0	50.0	58.6	58.4	1873-1882 bp	1.0	72.7