Hotspot

rpoB-trnC-GCA

LSC · 27302-28465 · 1164 bp

Back to Achimenes

Pi 0.00687
MarkerSeek score 62.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1164
variable_sites
8
pi
0.00687
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 ATTGCCTCATTTCCATCCCC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.9 58.1 1252 bp 1.0 79.1
rpoB-trnC-GCA_p2 ATTGCCTCATTTCCATCCCC AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.9 58.4 1234 bp 1.0 76.9
rpoB-trnC-GCA_p3 ATTGCCTCATTTCCATCCCC TTTGTTGATCAGGCGACACC 50.0 50.0 57.9 58.8 1271 bp 1.0 73.6
rpoB-trnC-GCA_p4 ATTGCCTCATTTCCATCCCC GACACCCGGATTTGAACTGG 50.0 55.0 57.9 58.8 1257 bp 1.0 73.1
rpoB-trnC-GCA_p5 CTCATTTCCATCCCCGAGC CCGGATTTGAACTGGGGAAA 57.9 50.0 58.0 58.1 1247 bp 1.0 71.8