Hotspot

atpH-atpI

LSC · 13974-15008 · 1035 bp

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Pi 0.00483

MarkerSeek score 60.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1035
variable_sites: 5
pi: 0.00483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCGATAGAAGCAAGCCCTA	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.9	58.3	1208-1209 bp	1.0	77.6
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.1	58.3	1169-1170 bp	1.0	77.4
atpH-atpI_p3	CCGATAGAAGCAAGCCCTAC	TCCCTGTCATGTTCCTTGGA	55.0	50.0	57.8	58.3	1207-1208 bp	1.0	77.0
atpH-atpI_p4	GATAGAAGCAAGCCCTACGG	TCCCTGTCATGTTCCTTGGA	55.0	50.0	57.8	58.3	1205-1206 bp	1.0	77.0
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	1186-1187 bp	1.0	76.6