Hotspot

trnK-UUU-rps16

LSC · 4207-5111 · 905 bp

Back to Achimenes

Pi 0.0383

MarkerSeek score 77.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 905
variable_sites: 34
pi: 0.0383
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	1026-1042 bp	1.0	82.6
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	1025-1041 bp	1.0	82.6
trnK-UUU-rps16_p3	TCAGTCGTGGTCTTCCAAAC	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	57.8	58.0	1111-1127 bp	1.0	81.8
trnK-UUU-rps16_p4	CAGTCGTGGTCTTCCAAACT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	57.8	58.0	1110-1126 bp	1.0	81.8
trnK-UUU-rps16_p5	TCTAGCCGCACTTAAAAGCC	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.3	58.0	1040-1056 bp	1.0	81.8