Hotspot

ndhF

SSC · 106547-108778 · 2232 bp

Back to Achillea

Pi 0.00157

MarkerSeek score 34.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 7
pi: 0.00157
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTAGCACTGGGTAGACCTCA	AGCTAAGATCCGGTGAATCG	55.0	50.0	57.9	57.2	2646-2711 bp	1.0	72.8
ndhF_p2	TCGTTGTGGTCGGACTCTAT	AGCTAAGATCCGGTGAATCG	50.0	50.0	58.2	57.2	2825-2890 bp	1.0	72.6
ndhF_p3	ATCAATTCGGTCGTTGTGGT	AGCTAAGATCCGGTGAATCG	45.0	50.0	57.8	57.2	2835-2900 bp	1.0	72.5
ndhF_p4	CTAGCACTGGGTAGACCTCA	CGAAAGAGCTAAGATCCGGT	55.0	50.0	57.9	57.1	2652-2717 bp	1.0	72.2
ndhF_p5	TCGTTGTGGTCGGACTCTAT	CGAAAGAGCTAAGATCCGGT	50.0	50.0	58.2	57.1	2831-2896 bp	1.0	72.0