Hotspot

ndhF-rpl32

SSC · 108779-109787 · 1009 bp

Back to Achillea

Pi 0.00363
MarkerSeek score 62.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1009
variable_sites
7
pi
0.00363
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTTTTTGTTGCCGTCGGAAA ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.8 57.9 1183-1213 bp 1.0 80.1
ndhF-rpl32_p2 CTTTTTGTTGCCGTCGGAAA AATGCCGCCCAATATCCTTT 45.0 45.0 57.8 57.9 1172-1202 bp 1.0 80.0
ndhF-rpl32_p3 AAGACTTTTTGTTGCCGTCG ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.5 57.9 1187-1217 bp 1.0 77.9
ndhF-rpl32_p4 ACGAAGACTTTTTGTTGCCG ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.5 57.9 1190-1220 bp 1.0 77.9
ndhF-rpl32_p5 CGAAGACTTTTTGTTGCCGT ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.5 57.9 1189-1219 bp 1.0 77.9