Hotspot

atpB-rbcL

LSC · 53805-54557 · 753 bp

Back to Achillea

Pi 0.00332

MarkerSeek score 61.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 753
variable_sites: 5
pi: 0.00332
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AACCCCAGAACTAGCAGGAT	ACCCCAGCTTTGAATCCAAC	50.0	50.0	58.0	58.4	823-834 bp	1.0	77.7
atpB-rbcL_p2	ACCCCAGAACTAGCAGGATT	ACCCCAGCTTTGAATCCAAC	50.0	50.0	58.0	58.4	822-833 bp	1.0	77.7
atpB-rbcL_p3	AACCCCAGAACTAGCAGGAT	CCCCAGCTTTGAATCCAACA	50.0	50.0	58.0	58.4	822-833 bp	1.0	77.7
atpB-rbcL_p4	ACCCCAGAACTAGCAGGATT	CCCCAGCTTTGAATCCAACA	50.0	50.0	58.0	58.4	821-832 bp	1.0	77.7
atpB-rbcL_p5	CTGGCGGAAAAGCTACATCT	ACCCCAGCTTTGAATCCAAC	50.0	50.0	58.0	58.4	893-904 bp	1.0	77.6