Hotspot

ndhC-trnV-UAC

LSC · 49725-50837 · 1113 bp

Back to Achillea

Pi 0.0018
MarkerSeek score 62.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1113
variable_sites
4
pi
0.0018
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTTGCTACTCGGAGCTA GAGAAGGTCTACGGTTCGAG 50.0 55.0 57.9 57.2 1245-1281 bp 1.0 73.8
ndhC-trnV-UAC_p2 TCCTTTGCTACTCGGAGCTA CGAGAAGGTCTACGGTTCGA 50.0 55.0 57.9 58.9 1246-1282 bp 1.0 73.1
ndhC-trnV-UAC_p3 ACTCGGAGCTAAAAATCCCG GAGAAGGTCTACGGTTCGAG 50.0 55.0 57.7 57.2 1236-1272 bp 1.0 72.4
ndhC-trnV-UAC_p4 TTCTCCGGTCCTTTGCTACT GAGAAGGTCTACGGTTCGAG 50.0 55.0 58.4 57.2 1253-1289 bp 1.0 72.0
ndhC-trnV-UAC_p5 ACTCGGAGCTAAAAATCCCG CGAGAAGGTCTACGGTTCGA 50.0 55.0 57.7 58.9 1237-1273 bp 1.0 71.7