Hotspot

trnL-UAA-trnF-GAA

LSC · 46923-47278 · 356 bp

Back to Achillea

Pi 0.00287

MarkerSeek score 52.20

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 356
variable_sites: 2
pi: 0.00287
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	424-432 bp	1.0	73.8
trnL-UAA-trnF-GAA_p2	ATCATGAGGGTTCAAGTCCC	TTTGAACTGGTGACACGAGG	50.0	50.0	56.9	58.1	434-442 bp	1.0	72.5
trnL-UAA-trnF-GAA_p3	AGAGTCCCGTTCTACATGTCA	TTTGAACTGGTGACACGAGG	47.6	50.0	58.2	58.1	507-515 bp	1.0	71.6
trnL-UAA-trnF-GAA_p4	GGTTCAAGTCCCTCTATCCC	TTTGAACTGGTGACACGAGG	55.0	50.0	56.7	58.1	426-434 bp	1.0	71.0
trnL-UAA-trnF-GAA_p5	GTTCAAGTCCCTCTATCCCC	TTTGAACTGGTGACACGAGG	55.0	50.0	56.7	58.1	425-433 bp	1.0	71.0