Hotspot

ycf3-trnS-GGA

LSC · 43618-44459 · 842 bp

Back to Achillea

Pi 0.00435
MarkerSeek score 63.10
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
842
variable_sites
7
pi
0.00435
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 976-1009 bp 1.0 67.2
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 977-1010 bp 1.0 67.2
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1014-1047 bp 1.0 66.9
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1015-1048 bp 1.0 66.9
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA AGTTCCAATGCTACGCCTTC 45.0 50.0 56.4 58.3 978-1011 bp 1.0 66.6