Hotspot

trnS-GCU-trnC-GCA

LSC · 8489-9231 · 743 bp

Back to Achillea

Pi 0.00561

MarkerSeek score 61.60

Primer available yes

Label trnS-GCU-trnC-GCA

Feature Metadata

label_name: trnS-GCU-trnC-GCA
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 8
pi: 0.00561
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	894-917 bp	1.0	78.8
trnS-GCU-trnC-GCA_p2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	876-899 bp	1.0	76.7
trnS-GCU-trnC-GCA_p3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.6	58.1	830-853 bp	1.0	75.0
trnS-GCU-trnC-GCA_p4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	895-918 bp	1.0	74.7
trnS-GCU-trnC-GCA_p5	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.6	58.4	812-835 bp	1.0	72.8