Hotspot

trnK-UUU-rps16

LSC · 4308-5132 · 825 bp

Back to Achillea

Pi 0.00324
MarkerSeek score 62.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
825
variable_sites
5
pi
0.00324
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TTGAAAAAGGTGCTCAGCCT 55.0 45.0 57.9 57.9 988-1048 bp 1.0 79.8
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC TTGAAAAAGGTGCTCAGCCT 55.0 45.0 57.9 57.9 987-1047 bp 1.0 79.8
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TTGAAAAAGGTGCTCAGCCT 50.0 45.0 58.2 57.9 927-987 bp 1.0 79.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTGAAAAAGGTGCTCAGCCT 50.0 45.0 58.2 57.9 926-986 bp 1.0 79.2
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC AAAAGGTGCTCAGCCTACAG 55.0 50.0 57.9 57.8 983-1043 bp 1.0 78.9