Hotspot

psbA

Genome · 559-1620 · 1062 bp

Back to Achatocarpus

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: Genome
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AAGAAATCGGTAATGCCCCT	AGAATTCGTGTGCTTGGGAG	45.0	50.0	56.9	58.2	1176 bp	1.0	69.5
psbA_p2	AAGAAATCGGTAATGCCCCT	AGAGAATTCGTGTGCTTGGG	45.0	50.0	56.9	58.2	1178 bp	1.0	69.5
psbA_p3	AGAAATCGGTAATGCCCCTT	AGAATTCGTGTGCTTGGGAG	45.0	50.0	56.9	58.2	1175 bp	1.0	69.5
psbA_p4	AGAAATCGGTAATGCCCCTT	AGAGAATTCGTGTGCTTGGG	45.0	50.0	56.9	58.2	1177 bp	1.0	69.5
psbA_p5	AGAAATCGGTAATGCCCCTT	GAGAATTCGTGTGCTTGGGA	45.0	50.0	56.9	58.2	1176 bp	1.0	69.5