Hotspot

atpF

Genome · 11654-12972 · 1319 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: Genome
length_bp: 1319
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTTCGTCTGCTCGAATGGTT	AGAAGTAGCAAAGAAGTGAA	45.0	35.0	57.8	52.0	1474 bp	1.0	40.0
atpF_p2	TTTCGTCTGCTCGAATGGTT	AGAAGTAGCAAAGAAGTGAAA	45.0	33.3	57.8	53.0	1474 bp	1.0	40.0
atpF_p3	TCGTCTGCTCGAATGGTTAC	AGAAGTAGCAAAGAAGTGAA	50.0	35.0	57.7	52.0	1472 bp	1.0	40.0
atpF_p4	TTTCGTCTGCTCGAATGGTT	AGCAAAGAAGTGAAATAATACAACGA	45.0	30.8	57.8	57.9	1468 bp	1.0	40.0
atpF_p5	TCGTCTGCTCGAATGGTTAC	AGAAGTAGCAAAGAAGTGAAA	50.0	33.3	57.7	53.0	1472 bp	1.0	40.0