Hotspot

atpA

Genome · 10063-11586 · 1524 bp

Back to Achatocarpus

Pi 0
MarkerSeek score 40.00
Primer available yes
Label atpA

Feature Metadata

label_name
atpA
feature_type
gene
region
Genome
length_bp
1524
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA_p1 GTTCAAATCCTATTGGACGCA GTTTGGTGCGATGAACGAAA 42.9 45.0 56.6 57.6 1742 bp 1.0 57.7
atpA_p2 GTTCAAATCCTATTGGACGCA CATGTTTGGTGCGATGAACG 42.9 50.0 56.6 58.4 1745 bp 1.0 57.7
atpA_p3 GTTCAAATCCTATTGGACGCA TATTGGCATGTTTGGTGCGA 42.9 45.0 56.6 58.5 1751 bp 1.0 57.1
atpA_p4 GTTCAAATCCTATTGGACGCA ATGTTTGGTGCGATGAACGA 42.9 45.0 56.6 58.5 1744 bp 1.0 57.0
atpA_p5 GTTCAAATCCTATTGGACGCA ATATTGGCATGTTTGGTGCG 42.9 45.0 56.6 57.4 1752 bp 1.0 56.2