Hotspot

trnT-UGU-trnL-UAA

Genome · 48017-48800 · 784 bp

Back to Achatocarpus

Pi 0.0194
MarkerSeek score 69.60
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
Genome
length_bp
784
variable_sites
15
pi
0.0194
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG AGCCAATTAAGTCCGTAGCG 55.0 50.0 57.9 58.1 857-939 bp 1.0 79.9
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT AGCCAATTAAGTCCGTAGCG 50.0 50.0 57.8 58.1 889-971 bp 1.0 79.0
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 845-927 bp 1.0 78.5
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG GCCAATTAAGTCCGTAGCGT 55.0 50.0 57.9 58.4 856-938 bp 1.0 77.6
trnT-UGU-trnL-UAA_p5 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 877-959 bp 1.0 77.6